FROM 23rd MAY – 4th JUNE 1999


INTRODUCTION: Dr Barbara Wilke’s visit to BANGKOK BIOMATERIAL CENTER (BBC) was a result of long efforts made by the Center towards the establishing and setup of a multipurpose Cell Culture Laboratory in the center with the support of the INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA). The Center has been working in conjunction with the IAEA since the last 5 years on this project (RCA/IAEA/THA/0/006-05). A milestone was achieved in this regard in 1998, when the Agency sent Prof. Rudiger Von Verson (from the German Institute of Cell and Tissue Culture, Berlin, Germany) as an expert to initiate the process of setting up the Cell Culture Laboratory. During this visit it was felt that the Center needed the expertise of an expert to teach the techniques of Cell Culture to the Scientists and technicians of the Center. It was also agreed that the Center needed support of the Agency for time to time visit by experts to oversee the progress and imparting advance training and technological know-how to the staffs of the Center. After the visit of a preliminary list of equipment and instruments required by the Center for an efficient setup was sent to Prof. Von Verson for his kind perusal and recommendation to the Agency.

In April 1999, IAEA intimated the center about the possibility of an expert visit by Dr. Barbara Wilke to the Center for the purpose of Training the Scientists and Technicians of the Center in the advance field of Keratinocyte Cell Culture. It was also intended that Dr. Wilke with her vast experience in the field of Keratinocyte Cell Culture would be able to guide the Center in the setting up of the Lab. The BANGKOK BIOMATERIAL CENTER welcomed this proposal of the Agency and after a hectic time schedule accommodation, the visit of Dr Wilke was made into a reality on the 23rd May 1999. Prof. Yongyudh Vajaradul – Director of the Center, received her in Bangkok.


1) To establish the Cell Culture Laboratory and try to make the method useful for clinical applications.

2) To setup a model tissue culture lab for service.

3) To fulfil the need for RCA/IAEA in the aspect of advance training   programme in the region.

4) Advance training center for Mahidol University.

5) To establish the network of Cell Culture Laboratory and users in Thailand.



2) RCA/IAEA/THA/0/006-05

3) Post Graduate School of Mahidol University

4) Ministry of Health – Thailand

5) Office of the Supreme Commander – Thailand

6) IAEA/325-RAS/7/008 THA Master Degree programme


1) Expert visit of Dr. Barbara Wilke

2) Network establishment

3) Laboratory Setup

4) Scientists and technician training

5) Master Degree Studies

6) Clinical applications and research


1) Regular budget of the BANGKOK BIOMATERIAL CENTER

2) Support from IAEA

3) Reimbursement from patients of working cost

4) Donations

Programme Schedule during the visit to Bangkok Biomaterial Center (BBC)

SUNDAY 23.5.99

17.00 hrs:

19.00 – 20.30 hrs


Reception at Don Muang Airport

Briefing about the background of the Cell Culture Laboratory


Prof. Yongyudh Vajaradul


MONDAY 24.5.99

 08.00 – 09.00 hrs

10.00 – 11.00 hrs

11.00 – 12.30 hrs

12.30 – 13.30 hrs

13.30 – 16.30 hrs



Welcome and Introduction at the Center

Briefing - Current status of Keratinocyte Cell Culture (KCC) Laboratory set-up at BANGKOK BIOMATERIAL CENTER (Prof.Yongyudh and Dr. Barbara discussing)

Input Lecture on Keratinocyte Cell Culture (students taking note)

Lunch & Discussions

Visit to Pharmacology Laboratory and preparation for the KCC processing technique. Check up available instruments, equipment’s, chemicals Reagents and other basic requirements


Prof. Yongyudh Vajaradul

Prof. Yongyudh Vajaradul


Dr. Barbara Wilke


Dr. Adisak

TUESDAY 25.5.99

09.00 – 12.30 hrs

12.30 – 13.30 hrs

13.30–16.00 hrs


Laboratory overview, setting and lecture at the BBC (students taking note)

Lunch & discussions

Keratinocyte Cell Culture theory and practice, input lecture


Dr. Barbara Wilke


Dr. Barbara Wilke


09.00 - 16.00 hrs


Specimen preparation for Keratinocyte Cell Culture at the pharmacology laboratory. On Hand training of the scientists, technicians and Master degree students on the first step to the Processing of skin specimen, sterility techniques And other basic pre-preparation requirements


Dr Barbara Wilke

THURSDAY 27.5.99


09.00 – 13.00 hrs


13.00 – 13.30 hrs

13.30 – 16.00 hrs


Discussion and planing with Mr. Reyaz Alam Qayami and Mr. Pisit regarding the existing Lab facilities, equipment’s, instruments at the Center. Formulating the Working Protocols and Procedure for Keratinocyte Cell Culture. Finalizing The list of chemicals, reagents, mediums and their Source with catalogue numbers and available brand names in the international market. Preparation of the Specimen for final demonstration at the Pharmacology Lab.(Dr. Barbara seeing the Gama Radiation facility at BBC)

Lunch & Discussions

Question and Answer Session of the last practical session. Lecture on the next step of working with respect to the processing of the Keratinocyte Cell Culture






FRIDAY 28.5.99

09.00 – 17.30 hrs


Lecture & Demonstration of next step in the preparation and processing of Keratinocyte Cell Culture at Pharmacology lab


Dr Barbara Wilke

SATURDAY 29.5.99

10.00 – 13.30 hrs


Question and Answer session of the last practical class followed by Lecture at the Center


Dr Barbara Wilke

TUESDAY 01.6.99

09.00 –17.00 hrs


Visit to Biotec laboratory and meeting on the possibilities of sharing of resources and facilities between the Center and Biotec (Meeting at Biotech) , (Dr. Barbara setting the microscope to view the KC) ,

(Prof. Yongyudh sees into the microscope for the Keratinocyte cells as Dr. Naresh and Dr. Barbara looks on)


Dr Nares D.


09.00 – 16.00 hrs


Demonstrations at pharmacology lab of the stages of culture. On hand training of the Scientists, technicians and master degree students of the techniques for changing of medium and microscopic observations of the growing stages of the cells and techniques of cell counting and various tips and tricks in this regard. (Dr.Barbara at work while students wait for their chance to see the cells) , (view of the Keratinocyte Cells taken from the microscope with the help of a digital camera - 1  ,  2   ,  3 )


Dr Barbara Wilke

THURSDAY 03.6.99

09.00 – 16.00 hrs


Meeting with Prof. Yongyudh and the Cell Culture Laboratory project team regarding the setup steps, Present situation, present limitations, future problems Quality control measures, environmental hazards, Equipment’s, instruments, availability of raw materials Like chemicals, reagents and mediums. Relevant protocols, followed by advance centers and the importance of Radiation sterilization of the cells. (Farewell , Prof Yongyudh presenting the momento to Dr. Barbara as Dr. Chaweewan looks on)
FRIDAY 04.6.99

09.00 – 13.00 hrs


13.00 – 13.30 hrs

13.30 – 16.30 hrs

16.30 – 18.30 hrs

18.30 – 19.30 hrs

19.30 - 20.30 hrs

20.30 – 21.30 hrs


Final observation of Keratinocyte Cells at the Laboratory. Last minute tips and tricks. Sample Testing of the scientists, technicians and master Degree students absorption and assimilation of the Training imparted. Final questions, answers and queries with regard to the last practical session and open to all doubts, queries at the Bangkok Biomaterial Center.

Lunch & Discussion

Summary & Sum-up at Bangkok Biomaterial Center with Prof. Yongyudh Vajaradul and the project members.

Final preparation of working instruction and working procedure protocols for Keratinocyte Cell Culture with Mr. Reyaz.

Last minute Tips & Tricks, discussion and preparation of the minutes with Mr. Reyaz

Farewell Dinner

Drop to Airport. (Mr. Reyaz and Dr. Barbara before see-off at the Airport)


Dr Barbara Wilke








CONCLUSION: The wisdom with respect to Keratinocyte Cell Culture gained from the visit of Dr Barbara Wilke was immense and cannot be described in simple words. The visit helped the center, it’s scientists, technicians and the master degree students to learn the advance technique of Keratinocyte Cell Culture. There was a good amount of practical overhauling of the process and every participant was satisfied and confident of being able to do the processing and preparation of the cells independently. This confidence in itself is a great landmark to the setting up of the Cell Culture Laboratory. The Center is thankful to IAEA and Dr Barbara for all the help, co-operation and dissemination of knowledge.


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